Peter Parker/Spider-Man is the eponymous protagonist. Spidey's game play is almost identical to that of its predecessor, whereby he has to go around and save any civilians that are in trouble, engage in combat with thugs and (in the Nintendo DS version) take part in 3 touch screen mini-games: The stroke game, the web draw and the arm/disarm games.
Noman Osborn/Green Goblin is the main antagonist and a playable character. Green Goblin's game play is slightly different. Unlike Venom who could be controlled via the touch screen, the Goblin is controlled with the d-pad and face buttons only. His game play involves destroying objects and fighting security guards & S.H.I.E.L.D. agents. The Goblin does not take part in any touch screen mini-games.
Exclusive to the DS version, both Spider-Man & Goblin have access to 4 special moves which appear on the bottom screen, landing hits on opponents increases a special meter that, once full, will allow them to use one of the 4 moves available.
The player will also have to keep an eye out for the Threat Assessment Meter (or TAM for short); when it appears, it will point towards where Spidey & Goblin have to go next and also tell the player how much time left they have to solve the problem. If they fail to sort out the problem when the TAM meter runs out, it'll be game over.