Nicktoons: Attack of the Toybots gameplay includes playing as two Nicktoons in some levels and piloting an operational mech suit (created by Jimmy Neutron) in others. Players journey through Bikini Bottom, Amity Park, the Ghost Zone (GBA version only), Fairy World, Pupununu Village (handheld version only), Retroville (handheld version only), and Calamitous' Lair (console version only), as well as the Toybot Factory, which is divided into multiple segments.