Medal of Honor takes place near the end of World War II (mid-1944 to mid-1945). The goal of the game is to complete various Office of Strategic Services (OSS) missions, such as rescuing an American pilot, going undercover to board and destroy a U-boat, recovering stolen art work, and sabotaging the Nazi war effort.
The game includes a split-screen deathmatch mode, pitting two players against each other in various maps. Players can unlock several secret characters after completing the game or through cheat codes, including notable historical figures such as Filipino patriot José Rizal and playwright William Shakespeare, as well as outlandish characters such as a German Shepherd and a dinosaur.