Penny's father has been captured by Calico and his organization in an attempt to construct a powerful weapon of untold power. Penny and Bolt travel to Italy in an effort to find out more, but they get nowhere other than learning of Calico's location in Belize.
Calico finally manages to capture Penny once she and Bolt find his temple base in the Yucatán jungle. To prove to Penny's father that she is alive and being held hostage, Calico gives Penny a cell phone with her father on the other line. Calico then attempts to flee the crumbling temple with his new captive but decides to leave her there with Bolt to die, saying, "As long as the professor believes she's alive, he will do what is asked and finish the weapon. Too bad we can't all have nine lives".
However, Penny and Bolt survive and discover Calico's new base in the Russian Arctic with a large rocket in the center. But upon entering, Penny notices micro-focusing mirrors and realizes that it is her father's satellite instead of a warhead. All too late as Calico seals the entrance and, to Bolt's horror, launches it with Penny inside screaming for help. Bolt then jumps onto the rocket and dismantles it in mid-flight, crashing it into the snow.
The two then venture to Calico's harbor in China (discovered by Penny by hacking into a computer system), where they discover a ship full of warheads. Just then, Calico arrives at the harbor, enraged to find Penny still alive, and even more so as he watches his prized freight ship sink to the bottom, thanks to Bolt, who then pursues Calico, escaping in his car to a nearby train station. He arrives at the station and boards his train, closely followed by Penny and her dog. Just as the duo finally corners the doctor, he slips into a hidden room and escapes on his private jet, though not before Penny secures a homing beacon onto the hull, leading them right to Calico's base.